Regulatory

Part:BBa_K3033001

Designed by: Philip Naderev P.Lagniton   Group: iGEM19_UM_Macau   (2019-10-12)


Arabad promoter

Derived from pBAD24 vector; Arabinose inducible promoter for protein expression. The promoter contains a site that is similar to the RNA polymerase recognition sites in the galactose and lactose operons. It also contains a region similar to the known cyclic AMP receptor protein binding sites in the galactose and lactose operons. Within the araBAD promoter there are potentially three distinct sites at which proteins may interact: these are sites for RNA polymerase, araC protein, and cyclic AMP receptor protein (CRP), respectively.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


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Categories
Parameters
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